Betzig shared the 2014 Nobel Prize in Chemistry for developing PALM, a single-molecule localization method that broke the optical diffraction limit, and subsequently invented lattice light-sheet and adaptive-optics microscopy to image subcellular dynamics in living organisms with minimal phototoxicity. His current work, split between Berkeley and Janelia, continues to push the spatial and temporal resolution of live-cell and developmental imaging beyond conventional limits.
Boecking leads the Molecular Machines Group and is acting director of the EMBL Australia Node in Single Molecule Science. The group reconstitutes molecular machines — clathrin coat disassembly, HIV capsid assembly and uncoating, pore-forming toxins — and watches them work one molecule at a time by TIRF, interferometric scattering (mass photometry) and fluorescence fluctuation methods, resolving short-lived intermediates that ensemble kinetics averages into invisibility. He trained originally in surface chemistry and biosensors with Gooding, which gives the group unusual competence in engineering the surfaces these assays run on. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the argument for single-molecule methods over ensemble ones is identical to the argument for pushing NV sensing below its pT/sqrt(Hz) ensemble regime: the interesting biology lives in heterogeneity and in transient states that averaging destroys. Strong methodological neighbour for a quantum-biosensing candidate.
Dhiman holds the professorship for Physical Chemistry of Supramolecular Systems at JGU and is affiliated with the Max Planck Graduate Center. Her group uses single-molecule and super-resolution fluorescence microscopy (SMLM/PAINT-type methods) to watch synthetic supramolecular polymers assemble, exchange monomers and age in real time -- i.e. applying the biological super-resolution toolkit to non-biological self-assembling matter, and toward bioinspired/adaptive systems that behave like living materials. Relative to the established NV-ensemble quantum-sensing playbook (DEER, nanoscale NMR, T1 relaxometry at pT/sqrt(Hz) ensemble sensitivity), this is a technique-driven inclusion: the emphasis is squarely on pushing spatial and temporal resolution of dye-based imaging past the ensemble limit, and it is a newer group where a postdoc would have room to shape the direction.
Hylkje Geertsema uses single-molecule super-resolution fluorescence microscopy (TIRF, SMLM, PALM/STORM) to study DNA replication dynamics. Her lab visualises and quantifies individual replication proteins at replication forks in living cells to understand the kinetics and fidelity of DNA copying. Research focuses on measuring spatiotemporal dynamics of protein assemblies during DNA metabolism with nanometre resolution.
Jacob Hoogenboom develops integrated correlative light and electron microscopy (CLEM) and molecular nanophotonic imaging. Research: (1) 3-in-1 microscopy combining light, electron beam, and ion beam for precise biological sample sectioning and protein localisation; (2) integrated CLEM for mapping proteins in cellular context; (3) single-molecule nanophotonic sensing using fluorescence. Relevant to advanced single-molecule biosensing approaches.
Lemke holds the chair of Synthetic Biophysics at JGU and is adjunct director at the Institute of Molecular Biology. The group's signature is combining genetic code expansion -- installing non-canonical amino acids so a dye can be clicked onto one chosen residue -- with single-molecule fluorescence: smFRET on intrinsically disordered proteins, super-resolution imaging of the nuclear pore complex and its FG-nucleoporin permeability barrier, and engineered membraneless organelles used as designer compartments in living cells. The result is single-molecule-resolution measurement of conformational dynamics and phase behaviour inside cells rather than in vitro. Relative to the established NV-ensemble quantum-sensing playbook (DEER, nanoscale NMR, T1 relaxometry at pT/sqrt(Hz) ensemble sensitivity), this is the strongest biosensing/advanced-microscopy host in Mainz: the labelling chemistry is precisely what a quantum-sensing postdoc would need to attach nanodiamonds or spin labels to a defined protein site, and the group already operates at the single-molecule sensitivity limit optically. Large, well-funded, internationally recruiting group.
Nobel laureate W. E. Moerner, who first detected and studied single molecules optically, now develops engineered point-spread-function and orientation-resolved single-molecule localization microscopy methods to track individual biomolecules and their rotational dynamics in cells with nanometer precision, well beyond the optical diffraction limit.
Monzel holds the biophysics/biophotonics professorship at Stuttgart's 2nd Institute of Physics. The group develops multiparametric imaging spectroscopy and high-resolution light microscopy -- combining super-resolution, fluorescence-fluctuation and lifetime-resolved methods -- to read out several observables at once in living cells and in biomimetic model membranes, and pairs this with magnetic nanoparticles used to apply and sense forces on cell-surface receptors (magnetogenetic control of signalling). Single-molecule analysis inside cells is an explicit focus. Relative to the established NV-ensemble quantum-sensing playbook (DEER, nanoscale NMR, T1 relaxometry at pT/sqrt(Hz) ensemble sensitivity), this is the closest thing at Stuttgart to a natural biological host for in-cell quantum sensing: the group already does single-molecule-resolution live-cell imaging and already works with magnetic nanoparticles, so nanodiamond relaxometry/thermometry would slot in with the readout stack it already runs. Relatively new appointment -- good moment to join.
Bernd Rieger works on computational super-resolution microscopy and live tissue imaging at the nanoscale. Research directions: (1) single-molecule localization microscopy (SMLM) algorithms and particle fusion; (2) 3D multi-label super-resolution imaging in tissue; (3) deep learning for biological image analysis. ERC grants; NL-BI Dutch Bioimaging consortium.
Shaevitz combines custom super-resolution and multifocal/3D imaging instrumentation with single-molecule tracking to make precision measurements of bacterial cell-shape mechanics, cytoskeletal dynamics (e.g. MreB), collective motility and pattern formation, and animal behavior quantification. His lab pioneered 3D live-cell imaging of bacterial shape during growth and continues to develop chromatic multifocal and localization-microscopy instrumentation in collaboration with the Yang and Gregor labs.