Ananthanarayanan was awarded the Royal Microscopical Society Life Sciences Award in 2025 for the use of novel microscopies in cell biology. Her group images individual motor proteins — dynein, kinesin — and the mitochondria they transport, in living cells, at single-molecule sensitivity, combining light-sheet and TIRF-class imaging with particle tracking to ask how organelle positioning and mitochondrial dynamics are controlled. The methodological emphasis is on getting single-molecule sensitivity inside a live cell rather than in vitro, which is the hard version of the problem. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — this is the closest thing at UNSW to a biological end-user for an in-cell quantum sensor: the mitochondrial systems she studies are precisely where NV nanodiamond thermometry and free-radical relaxometry at pT/sqrt(Hz) have been aimed, and she has the live-cell imaging infrastructure to validate any such measurement independently.

Bain develops advanced laser spectroscopy and super-resolution microscopy techniques for biological applications. Research directions: (1) Femtosecond time-resolved STED (stimulated emission depletion) — combining sub-diffraction spatial resolution with picosecond time resolution to study FRET dynamics in live cells with both spatial and lifetime precision; (2) Time-resolved polarized fluorescence — probing orientation distributions and rotational dynamics of fluorophores; (3) CW STED fluorescence lifetime reconstruction — lower-photodose STED for longer live-cell imaging; (4) Single-molecule FRET to study protein-protein interactions; (5) Single-particle tracking of membrane receptors relevant to viral entry and cancer signaling. Former PhD students include Siân Culley (now King's College, SMLM).

Bell's group uses DNA nanotechnology and advanced optical microscopy for single-molecule biosensing. Research directions: (1) DNA-based biosensing — DNA origami structures as programmable biosensing platforms; using structural switching of DNA nanodevices to sense specific biomolecules with single-molecule sensitivity; (2) Super-resolution microscopy with DNA — DNA-PAINT and FRET-based single-molecule localization for mapping molecular architectures in cells; (3) Solid-state nanopores — DNA-threaded through nanopores as a precision biosensor for protein identification and force measurement; (4) Multiplexed single-molecule detection — combining DNA-based sensors with optical readout for parallel biomolecule profiling. New group established at UCL, strong biosensing focus.

Berry studies rotary molecular motors, especially the bacterial flagellar motor, using novel forms of light microscopy (laser dark-field microscopy, back-focal-plane laser interferometry, optical and magnetic tweezers) to track sub-micron handles with nanometre and sub-millisecond resolution, revealing how these nanoscale engines are built, controlled and generate torque.

Boecking leads the Molecular Machines Group and is acting director of the EMBL Australia Node in Single Molecule Science. The group reconstitutes molecular machines — clathrin coat disassembly, HIV capsid assembly and uncoating, pore-forming toxins — and watches them work one molecule at a time by TIRF, interferometric scattering (mass photometry) and fluorescence fluctuation methods, resolving short-lived intermediates that ensemble kinetics averages into invisibility. He trained originally in surface chemistry and biosensors with Gooding, which gives the group unusual competence in engineering the surfaces these assays run on. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the argument for single-molecule methods over ensemble ones is identical to the argument for pushing NV sensing below its pT/sqrt(Hz) ensemble regime: the interesting biology lives in heterogeneity and in transient states that averaging destroys. Strong methodological neighbour for a quantum-biosensing candidate.

Studies co-translational protein folding using time-resolved single-molecule fluorescence spectroscopy synergistically combined with NMR and single-particle cryo-EM.

Curmi is a structural and single-molecule biophysicist whose most-cited work is on the light-harvesting antenna proteins of cryptophyte algae, where he and collaborators reported long-lived electronic coherence at ambient temperature — one of the founding results of the quantum-biology field and still one of its most argued-over. His group determines the structures of these antenna complexes and engineers them, and separately works on protein-based molecular motors and on single-molecule fluorescence and FRET measurements of conformational dynamics. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — Curmi supplies the biological systems in which quantum coherence is actually claimed to matter; a pT/sqrt(Hz)-class spin sensor capable of watching radical-pair or exciton dynamics in situ would be aimed at exactly the questions his structures raise. Preferred attribute present: genuine quantum-biology substrate rather than a quantum-flavoured metaphor.

Research focuses on quantum dynamics and excited-state reactivity in biological and synthetic light-harvesting systems. Discovered long-lived quantum coherence in photosynthetic light-harvesting complexes (FMO, 2007). Develops 2D electronic spectroscopy techniques to probe excitonic transport, open quantum systems, and photochemical reaction dynamics on femtosecond timescales. Director NSF QuBBE; co-director Berggren Center for Quantum Biology and Medicine.

Galland leads LQNO at EPFL investigating light-matter interactions in nano-structures and the quantum regime. Research directions: (1) NV centers in diamond for quantum sensing — spectroscopy of NV spin states in ultra-thin diamond membranes, development of diamond nanophotonic platforms for enhanced sensing sensitivity; collaboration on quantum sensing with color centers; (2) Plasmonic nanocavities — few-nm gap junctions enhance Raman scattering by ×10^9, enabling single-molecule vibrational spectroscopy and coherent control; ultrafast and single-photon detection of coherent phonon dynamics; (3) 2D heterostructure photonics — entangled photon pair generation enhanced by TMD heterostructures; valley-polarized exciton sources; (4) Optical frequency conversion for quantum applications. SNSF-funded professor, internationally recognized for molecular optomechanics and carbon nanotube quantum optics.

Gambin was the first EMBL Australia group leader appointed to Single Molecule Science. His signature method combines cell-free protein expression with two-colour single-molecule coincidence and fluctuation spectroscopy, which sidesteps purification entirely: proteins are expressed, labelled and measured in lysate, an order of magnitude faster than conventional interaction assays. The biology is protein self-association and aggregation — alpha-synuclein in Parkinson's, cardiac and muscular disease proteins — where the size distribution of oligomers, not the mean, is the quantity of interest. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the conceptual overlap with quantum biosensing is the insistence on distributions over averages, and his aggregation systems (paramagnetic-species-generating, redox-active amyloid) are a plausible target for T1-relaxometry-based NV detection at pT/sqrt(Hz) in the near term.