Emmanuel Beaurepaire (DR1 CNRS, LOB/École Polytechnique) is a pioneer of multiphoton and harmonic generation deep-tissue microscopy. Research: (1) two-photon excited fluorescence (2PEF) and three-photon deep-tissue brain imaging; (2) second-harmonic generation (SHG) and third-harmonic generation (THG) label-free imaging of collagen, myosin, myelin; (3) multimodal 3-photon light-sheet microscopy with ultrafast lasers; (4) metabolic imaging using FLIM/NADH. Key LOB permanent staff (May 2024). Active collaboration with LCF/Lasers group on ultrafast laser development.

Garcia combines light-sheet and single-molecule fluorescence imaging with quantitative modeling to measure transcriptional dynamics in living Drosophila embryos in real time, quantifying how individual promoters and enhancers make fast, precise decisions during development. The group is actively recruiting postdocs interested in physical biology and quantitative live imaging.

Gregor's Laboratory for the Physics of Life builds custom quantitative microscopes (single-objective oblique-plane light-sheet, multicolor live-imaging, single-molecule transcription imaging) to make precision, physics-style measurements of gene expression, morphogen gradients, and chromatin dynamics in living Drosophila embryos and mammalian gastruloids. He is actively recruiting PhD students and postdocs with expertise in super-resolution imaging, nonlinear/ultrafast optics, and instrumentation development.

Willy Supatto (DR2 CNRS, LOB) develops ultrafast two-photon light-sheet microscopy for quantitative in vivo imaging of embryo development and tissue dynamics. Research: (1) two-photon SPIM (light-sheet) for volumetric live imaging in zebrafish embryos; (2) SHG imaging of fibrillar proteins; (3) polarization-THG microscopy of structural anisotropy; (4) photodamage in multiphoton imaging. Part of key LOB team with Beaurepaire.