Emmanuel Beaurepaire (DR1 CNRS, LOB/École Polytechnique) is a pioneer of multiphoton and harmonic generation deep-tissue microscopy. Research: (1) two-photon excited fluorescence (2PEF) and three-photon deep-tissue brain imaging; (2) second-harmonic generation (SHG) and third-harmonic generation (THG) label-free imaging of collagen, myosin, myelin; (3) multimodal 3-photon light-sheet microscopy with ultrafast lasers; (4) metabolic imaging using FLIM/NADH. Key LOB permanent staff (May 2024). Active collaboration with LCF/Lasers group on ultrafast laser development.

Develops second- and third-harmonic generation (SHG/THG) nonlinear optical microscopy to image collagen and other non-centrosymmetric structural proteins label-free in tissue, with applications to cancer diagnosis and fibrosis, pushing spatial/orientational resolution of structural imaging in intact tissue.

Marie-Claire Schanne-Klein (DR1 CNRS, LOB) specializes in polarized SHG and THG microscopy for structural tissue imaging. Research: (1) polarimetric SHG imaging of collagen fibril organization — molecular orientation mapping; (2) THG microscopy for myelin and red blood cell imaging; (3) structural and functional label-free imaging of connective tissues; (4) multi-scale SHG/THG analysis of biopolymer structure. SHG expert in LOB.

Willy Supatto (DR2 CNRS, LOB) develops ultrafast two-photon light-sheet microscopy for quantitative in vivo imaging of embryo development and tissue dynamics. Research: (1) two-photon SPIM (light-sheet) for volumetric live imaging in zebrafish embryos; (2) SHG imaging of fibrillar proteins; (3) polarization-THG microscopy of structural anisotropy; (4) photodamage in multiphoton imaging. Part of key LOB team with Beaurepaire.