Brasselet is a CNRS researcher at Institut Fresnel developing polarization- and orientation-resolved fluorescence microscopy, using controlled excitation and detection polarization states to map the 3D orientation and organization of fluorescent probes and biomolecular assemblies (e.g. lipid order, amyloid and cytoskeletal structures) at and beyond the single-molecule level, including recent work on the mathematical foundations of polarimetric microscopy.

Rigneault leads the MOSAIC team at Institut Fresnel, developing label-free nonlinear optical microscopy (CARS/SRS) for chemically-specific imaging of lipids and biomolecules in tissue, and pioneering lensless, hair-thin fiber-bundle endoscopes based on wavefront control for minimally invasive deep-tissue and in vivo biological imaging. He holds 17 patents in optical engineering and molecular spectroscopy for the life sciences.

Sentenac develops computational super-resolution fluorescence microscopy at Institut Fresnel, notably Random Illumination Microscopy (RIM), which reconstructs sub-diffraction images from the statistics (variance) of many speckle-illuminated acquisitions without requiring photoswitchable probes, along with the underlying inverse-problem theory that establishes its resolution limits and robustness for live and thick-sample imaging.