Description: Rapid cryo-plunging or high-pressure freezing of samples at defined, ultrafast time delays after a stimulus (e.g. drug addition) to recover the temporal dynamics of nanoscale cell-signaling motions for subsequent electron or super-resolved optical imaging.
Prigozhin develops multicolor electron microscopy using cathodoluminescent nanoprobe protein tags and time-resolved cryo-vitrification methods to capture the nanoscale, sub-second dynamics of GPCR signaling and biomolecular condensate formation, aiming to add molecular-scale color and temporal resolution to electron microscopy's inherent nanoscale spatial resolution.