Rueda leads a single-molecule imaging group (jointly at Imperial and the MRC London Institute of Medical Sciences) that combines single-molecule FRET, fluorogenic RNA aptamer imaging and optical tweezers to reveal the structural dynamics of RNA folding/splicing, CRISPR-Cas9 target search and off-target activity, and chromatin-remodelling complexes; the aptamer-imaging technology has been spun out as the startup Irida.