Description: Combinatorial, stochastic genetic expression of multiple fluorescent proteins in neurons so that individual cells and their processes can be distinguished by color for circuit tracing.
Lichtman invented the multicolor 'Brainbow' fluorescent labeling method and pioneered large-scale, automated serial-section electron microscopy to reconstruct complete synaptic wiring diagrams (connectomes) of neural tissue, pushing spatial resolution and scale together to map circuit-level brain structure.