Technique - (12) Two-photon fluorescence microscopy

Type: Experimental

Description: Non-linear laser excitation providing intrinsic optical sectioning for deep-tissue and in vivo imaging of labeled structures.

Department(s)/lab(s): Bioengineering | Schultz Neurotechnology Group @ Imperial
Summary:

Schultz uses two-photon calcium imaging and other optical neurotechnology to study neural population activity in vivo, with application to understanding circuit dysfunction in neurodegenerative disease and to brain-machine interfaces.

Department(s)/lab(s): Princeton Neuroscience Institute | Tank Lab @ Princeton
Summary:

Tank is a pioneer of two-photon laser-scanning microscopy for imaging calcium dynamics in dendrites and neural circuits in vivo, and co-directs the Bezos Center for Neural Circuit Dynamics, which develops large-scale optical recording instrumentation combined with rodent virtual-reality systems to study persistent neural activity and short-term memory. His group's methodological contributions to cellular-resolution optical imaging underpin much of modern systems neuroscience.